rhesus monkey kidney cell line llc mk2  (ATCC)

Journal: Virologica Sinica

Article Title: Metatranscriptomics uncovers host immune and microbiome signatures specific to and shared between human metapneumovirus and respiratory syncytial virus infections in children

doi: 10.1016/j.virs.2026.03.002

Figure Lengend Snippet: Differential IRGs and ISGs expressed during hMPV infection. A Heatmap of differentially expressed IRGs and ISGs with P values of < 0.05 in the hMPV group versus the control group and the RSV group. B Detailed expression levels of IRGs and ISGs with P values < 0.05 in the three groups. Statistical analysis was performed with an unpaired t -test. P values of 0.05 (∗), 0.01 (∗∗), and 0.001 (∗∗∗) were considered significant; NS: not significant. C Real-time PCR quantification of selected IRGs in infected and control cells at 24, 48, and 72 h postinfection. RSV and hMPV were propagated in LLC-MK2 cells at a multiplicity of infection (MOI) of 0.1. N = 3. Statistical analysis was performed with an unpaired t -test. P values < 0.05 (∗), < 0.01 (∗∗), and < 0.0001 (∗∗∗∗) were considered significant. The bars in the figure panels show the means and standard errors of the means (SEMs).

Article Snippet: Adult rhesus monkey kidney cells (LLC-MK2) (CCL-7) were obtained from ATCC (Manassas, VA, USA).

Techniques: Infection, Control, Expressing, Real-time Polymerase Chain Reaction

Journal: Journal of Virology

Article Title: A small-molecule HSP90 inhibitor, NVP-HSP990, alleviates rotavirus infection

doi: 10.1128/jvi.01883-25

Figure Lengend Snippet: Cytotoxicity and inhibitory effects on RV replication of different HSP90 inhibitors. ( A ) Plots for cell viability of MA104, Caco-2, and HT-29 cells after treatment with NVP-HSP990, GA, or 17-AAG at indicated concentrations for 24 h. Cell viability was tested using the CCK-8 assay. ( B ) Plots for RV (Wa and SA11 strains) inhibition in MA104, Caco-2, and HT-29 cells after treatment with NVP-HSP990, GA, or 17-AAG at indicated concentrations for 24 h. RV replication was tested by PFA, and IC 50 values are indicated at the top of each plot. The experiments were performed in triplicate, and the data are presented as mean ± SEM and are representative of four ( A ) and two ( B ) independent experiments. ns, not significant; * P < 0.05, ** P < 0.01, **** P < 0.0001 (two-way ANOVA).

Article Snippet: Rhesus monkey embryo kidney cell line MA104 cells (ATCC: CRL-2378.1) were provided by Dr. Elschner (Friedrich-Loeffler-Institute).

Techniques: CCK-8 Assay, Inhibition

Journal: Journal of Virology

Article Title: A small-molecule HSP90 inhibitor, NVP-HSP990, alleviates rotavirus infection

doi: 10.1128/jvi.01883-25

Figure Lengend Snippet: NVP-HSP990 inhibited MAPK activation and facilitated expression of tight junction-associated proteins in intestinal cells. ( A ) MA104, Caco-2, and HT-29 cells were mock-infected with PBS or infected with RV Wa or SA11 strains (MOI = 3), followed by treatment of 100 nM HSP990 (+) or an equal volume of DMSO as a control (−) for 20 h. Then the infected cells were harvested for WB analysis of MAPK components. ( B ) Caco-2 cells were mock-infected with PBS, treated with 1 µM C16-PAF(C16), or infected with RV Wa or SA11 strains (MOI = 3), and then treated with 100 nM HSP990 (+) or DMSO as a control (−) for 20 h. The infected cells were harvested for WB analysis of MAPK components. ( C ) Caco-2 cells were mock-infected with PBS or infected with RV Wa or SA11 strains (MOI = 3), and then treated with 100 nM HSP990 (+) or DMSO as control (−) for 20 h. Then the infected cells were harvested for WB analysis of tight junction-associated proteins. Data are representative of three ( A ) and two ( B and C ) independent experiments.

Article Snippet: Rhesus monkey embryo kidney cell line MA104 cells (ATCC: CRL-2378.1) were provided by Dr. Elschner (Friedrich-Loeffler-Institute).

Techniques: Activation Assay, Expressing, Infection, Control